Journal: Journal of Clinical Medicine

Article Title: Adiponectin Associates with Rheumatoid Arthritis Risk in Overweight and Obesity Independently of Other Adipokines

doi: 10.3390/jcm10132791

Figure Lengend Snippet: Baseline characteristics of the nested case-control cohort from the SOS study.

Article Snippet: Serum leptin, resistin, and visfatin levels were measured using Human Leptin Quantikine ELISA Kit (DLP00, Bio-Techne, Minneapolis, MN, USA), Human Resistin Quantikine ELISA Kit (DRSN00, Bio-Techne, Minneapolis, MN, USA), and Nampt (Visfatin/PBEF) human ELISA Kit (AG-45A-0006YEK-KI01, AdipoGen Life Sciences, San Diego, CA, USA) respectively, between April and June 2018 at the University of Gothenburg, Sweden.

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Adiponectin Associates with Rheumatoid Arthritis Risk in Overweight and Obesity Independently of Other Adipokines

doi: 10.3390/jcm10132791

Figure Lengend Snippet: Baseline characteristics of the nested case-control cohort from the Medical Biobank of Northern Sweden.

Article Snippet: Serum leptin, resistin, and visfatin levels were measured using Human Leptin Quantikine ELISA Kit (DLP00, Bio-Techne, Minneapolis, MN, USA), Human Resistin Quantikine ELISA Kit (DRSN00, Bio-Techne, Minneapolis, MN, USA), and Nampt (Visfatin/PBEF) human ELISA Kit (AG-45A-0006YEK-KI01, AdipoGen Life Sciences, San Diego, CA, USA) respectively, between April and June 2018 at the University of Gothenburg, Sweden.

Techniques: Northern Blot

Journal: Journal of Clinical Medicine

Article Title: Adiponectin Associates with Rheumatoid Arthritis Risk in Overweight and Obesity Independently of Other Adipokines

doi: 10.3390/jcm10132791

Figure Lengend Snippet: Multivariable conditional logistic regression analysis for RA in the nested case-control cohort from the SOS study.

Article Snippet: Serum leptin, resistin, and visfatin levels were measured using Human Leptin Quantikine ELISA Kit (DLP00, Bio-Techne, Minneapolis, MN, USA), Human Resistin Quantikine ELISA Kit (DRSN00, Bio-Techne, Minneapolis, MN, USA), and Nampt (Visfatin/PBEF) human ELISA Kit (AG-45A-0006YEK-KI01, AdipoGen Life Sciences, San Diego, CA, USA) respectively, between April and June 2018 at the University of Gothenburg, Sweden.

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Adiponectin Associates with Rheumatoid Arthritis Risk in Overweight and Obesity Independently of Other Adipokines

doi: 10.3390/jcm10132791

Figure Lengend Snippet: Multivariable conditional logistic regression analysis for RA risk (A) and logistic regression analyses after stratifying for BMI (B and C) in the Medical Biobank of Northern Sweden cohort.

Article Snippet: Serum leptin, resistin, and visfatin levels were measured using Human Leptin Quantikine ELISA Kit (DLP00, Bio-Techne, Minneapolis, MN, USA), Human Resistin Quantikine ELISA Kit (DRSN00, Bio-Techne, Minneapolis, MN, USA), and Nampt (Visfatin/PBEF) human ELISA Kit (AG-45A-0006YEK-KI01, AdipoGen Life Sciences, San Diego, CA, USA) respectively, between April and June 2018 at the University of Gothenburg, Sweden.

Techniques: Northern Blot

Journal: Journal of Clinical Medicine

Article Title: Adiponectin Associates with Rheumatoid Arthritis Risk in Overweight and Obesity Independently of Other Adipokines

doi: 10.3390/jcm10132791

Figure Lengend Snippet: Baseline characteristics of the cohort from the Medical Biobank of Northern Sweden stratified by BMI.

Article Snippet: Serum leptin, resistin, and visfatin levels were measured using Human Leptin Quantikine ELISA Kit (DLP00, Bio-Techne, Minneapolis, MN, USA), Human Resistin Quantikine ELISA Kit (DRSN00, Bio-Techne, Minneapolis, MN, USA), and Nampt (Visfatin/PBEF) human ELISA Kit (AG-45A-0006YEK-KI01, AdipoGen Life Sciences, San Diego, CA, USA) respectively, between April and June 2018 at the University of Gothenburg, Sweden.

Techniques: Northern Blot

Journal: International Journal of Fertility & Sterility

Article Title: In Vitro Fertilization, Levels of Pro-Inflammatory Factors and Lipid Peroxidation

doi:

Figure Lengend Snippet: The mean ± SE and P values of the control and IVF groups

Article Snippet: The intra-assay and inter-assay coefficients of variation (CV) were 5.5 and 7.0%, respectively, using the AssayMax Human Resistin ELISA kit (Assaypro, USA).

Techniques:

Journal: International Journal of Fertility & Sterility

Article Title: In Vitro Fertilization, Levels of Pro-Inflammatory Factors and Lipid Peroxidation

doi:

Figure Lengend Snippet: The mean ± SE and P values of the non-pregnant and pregnant women in pre-IVF and post-IVF groups

Article Snippet: The intra-assay and inter-assay coefficients of variation (CV) were 5.5 and 7.0%, respectively, using the AssayMax Human Resistin ELISA kit (Assaypro, USA).

Techniques:

Journal: PLOS Genetics

Article Title: Single-cell RNA profiling reveals classification and characteristics of mononuclear phagocytes in colorectal cancer

doi: 10.1371/journal.pgen.1011176

Figure Lengend Snippet: Antibodies information.

Article Snippet: RETN Polyclonal antibody , proteintech , Cat No. 18170-1-AP.

Techniques:

Journal: Arquivos Brasileiros de Cardiologia

Article Title: Obesity, Serum Resistin and Leptin Levels Linked to Coronary Artery Disease

doi: 10.5935/abc.20160134

Figure Lengend Snippet: Demographic and chemical characteristics of subjects

Article Snippet: Levels of serum resistin and leptin were measured by enzyme-linked immune-sorbent assay (ELISA) with commercial kits: [HUMAN resistin ELISA kit (Cat.No:EK0581; Boster biological technology; 40459 Encyclopedia Circle, Fermont, CA 94538, USA], and [HUMAN leptin ELISA kit (Cat. No: RD 191001100, USA).The serum samples were immediately frozen at -70oC until analysis.

Techniques:

Journal: Arquivos Brasileiros de Cardiologia

Article Title: Obesity, Serum Resistin and Leptin Levels Linked to Coronary Artery Disease

doi: 10.5935/abc.20160134

Figure Lengend Snippet: Correlation between resistin with body mass index (BMI) (A) and waist circumference (WC) (B).

Article Snippet: Levels of serum resistin and leptin were measured by enzyme-linked immune-sorbent assay (ELISA) with commercial kits: [HUMAN resistin ELISA kit (Cat.No:EK0581; Boster biological technology; 40459 Encyclopedia Circle, Fermont, CA 94538, USA], and [HUMAN leptin ELISA kit (Cat. No: RD 191001100, USA).The serum samples were immediately frozen at -70oC until analysis.

Techniques:

Journal: Journal of Translational Medicine

Article Title: Plasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling pattern

doi: 10.1186/s12967-019-2060-7

Figure Lengend Snippet: Forward and reverse primers used in real-time PCR

Article Snippet: Leptin concentrations were assessed with Human Leptin ELISA kit (Merck Millipore, MA, US), adiponectin with Human Adiponectin Elisa kit (Merck Millipore, MA, US), resistin with Human Resistin Elisa kit (Merck Millipore, MA, US).

Techniques:

Journal: Journal of Translational Medicine

Article Title: Plasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling pattern

doi: 10.1186/s12967-019-2060-7

Figure Lengend Snippet: Concentration of adipokines in plasma measured by ELISA (n = 55); differences between the groups were analyzed with one-way analysis of variance (ANOVA) followed by Newman-Keuls post hoc test; **indicates p < 0.01

Article Snippet: Leptin concentrations were assessed with Human Leptin ELISA kit (Merck Millipore, MA, US), adiponectin with Human Adiponectin Elisa kit (Merck Millipore, MA, US), resistin with Human Resistin Elisa kit (Merck Millipore, MA, US).

Techniques: Concentration Assay, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Journal of Translational Medicine

Article Title: Plasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling pattern

doi: 10.1186/s12967-019-2060-7

Figure Lengend Snippet: Stepwise regression analysis of adipokines and echocardiographic heart parameters

Article Snippet: Leptin concentrations were assessed with Human Leptin ELISA kit (Merck Millipore, MA, US), adiponectin with Human Adiponectin Elisa kit (Merck Millipore, MA, US), resistin with Human Resistin Elisa kit (Merck Millipore, MA, US).

Techniques: Clinical Proteomics

Journal: bioRxiv

Article Title: Granulocyte Derived Resistin Inhibits Monocyte Maturation and Induces Immune Suppression in CMML

doi: 10.1101/2025.03.03.640303

Figure Lengend Snippet: A: Normalised expression levels of RETN in granulocytes, CD34+ HSPCs, CD14+ monocytes and whole PBMNCs from CMML patients and healthy (young and/or old) controls , , . B. Relative expression levels of RETN in granulocytes sorted from different myeloid cancer patients . aCML: atypical CML, MPN-U: MPN-unclassifiable, CNL: Chronic Neutrophilic Leukaemia, MDS/MPN-U: MDS/MPN-unclassifiable. C. Resistin protein (ng/ml) levels in healthy (N=14) and CMML (N=27) plasma samples as determined by ELISA. D. Pearson correlation between CMML granulocyte RETN expression and matched plasma resistin concentration. E & F. Kaplan-Meier overall (E) and AML-free survival plot (F) of CMML patients stratified by plasma resistin levels (High resistin N=9; Low resistin N=18). Error bars = ± SEM; ns, no significance; ****, P<0.0001.

Article Snippet: The resistin concentration was measured in the PPP supernatant using the Human Resistin Standard ABTS ELISA Development Kit (Peprotech, 900-K235), according to manufacturer’s protocol.

Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: bioRxiv

Article Title: Granulocyte Derived Resistin Inhibits Monocyte Maturation and Induces Immune Suppression in CMML

doi: 10.1101/2025.03.03.640303

Figure Lengend Snippet: A. Representative flow cytometry plots showing the surface marker expression of CD14 and CD16 on freshly isolated healthy monocytes (0hrs) and monocytes treated with increase in concentration of resistin or control GFP for 24 hours (N=5). B & D. Bar charts showing average percentage of CD14+ or CD14+CD16+ populations under indicated conditions. C. Representative flow cytometry plots showing the surface marker expression of CD14 and CD16 on monocytes incubated with or without TLR4 neutralising antibody (TLR4Ab) prior to exposure with resistin or GFP control for 24 hours (N=4). E. Relative expression levels of RETN in three different types of human healthy monocytes . F. Representative brightfield microscope images of healthy monocytes/macrophages morphology following 6-day differentiation of monocytes treated either with 250 ng/ml of GFP control or resistin (Left). Blue arrows = attached macrophages; red arrows = suspended monocytes; scale bar 100 µm. Quantification cell confluency after 6 days treatment of monocytes either with GFP control or resistin (right). G. Percentage of live cells in suspension cultures of monocyte differentiation, treated either with GFP control or resistin. Error bars = ± SEM; ns, no significance; *, P<0.05; **, P<0.01; ***, P<0.001.

Article Snippet: The resistin concentration was measured in the PPP supernatant using the Human Resistin Standard ABTS ELISA Development Kit (Peprotech, 900-K235), according to manufacturer’s protocol.

Techniques: Flow Cytometry, Marker, Expressing, Isolation, Concentration Assay, Control, Incubation, Microscopy, Suspension

Journal: bioRxiv

Article Title: Granulocyte Derived Resistin Inhibits Monocyte Maturation and Induces Immune Suppression in CMML

doi: 10.1101/2025.03.03.640303

Figure Lengend Snippet: A. Schematic diagram depicting transcriptome study investigating downstream regulators of resistin or LPS on healthy monocytes. Monocytes treated recombinant protein GFP protein and freshly isolated monocytes were used as controls. B. Principal component analysis of the RNA-seq data. C. GO analysis of genes induced by resistin at 6 and 24 hrs together as compared with respective time point controls. Top ten significant pathways are shown. D & E. GSEA plots showing relative enrichment of indicated gene sets in different comparisons as listed. F. Heatmap showing expression of top30 DEGs across different comparisons.

Article Snippet: The resistin concentration was measured in the PPP supernatant using the Human Resistin Standard ABTS ELISA Development Kit (Peprotech, 900-K235), according to manufacturer’s protocol.

Techniques: Recombinant, Isolation, RNA Sequencing, Expressing

Journal: bioRxiv

Article Title: Granulocyte Derived Resistin Inhibits Monocyte Maturation and Induces Immune Suppression in CMML

doi: 10.1101/2025.03.03.640303

Figure Lengend Snippet: A. Venn diagram showing overlap of genes upregulated following 6 and 24 hrs treatment with either resistin or LPS in comparison with their respective time point controls. B. Venn diagram showing overlap of genes uniquely upregulated by resistin but not by LPS with genes that are upregulated in CMML monocytes . C. Relative expression levels of SEMA4A in monocytes treated with either resistin or control GFP protein. D. Relative expression levels of SEMA4A in CMML and healthy monocytes. E. Mean fluorescence intensity (MFI) of SEMA4A on healthy monocytes treated with either control GFP or increase in concentration of resistin for 24 hrs (N=4). F. Kaplan-Meier overall (left) and AML-free (right) survival plot of CMML patients stratified by SEMA4A expression levels (N=11 High; N=79 Low) G. GSEA plots showing relative enrichment of indicated gene sets in resistin (6 hrs) treated monocytes in comparison with the controls. Error bars = ± SEM; ns, no significance; *, P<0.05; **, P<0.01.

Article Snippet: The resistin concentration was measured in the PPP supernatant using the Human Resistin Standard ABTS ELISA Development Kit (Peprotech, 900-K235), according to manufacturer’s protocol.

Techniques: Comparison, Expressing, Control, Fluorescence, Concentration Assay

Journal: bioRxiv

Article Title: Granulocyte Derived Resistin Inhibits Monocyte Maturation and Induces Immune Suppression in CMML

doi: 10.1101/2025.03.03.640303

Figure Lengend Snippet: A. Ratio of Th2/Th1 cells in ex vivo cultures of whole- or CD14 depleted-PBMNCs treated with either GFP or resistin for 9 days (N=6). B. Th2/Th1 ratio from healthy (N=10) and CMML (N=8) peripheral blood (PB). C. Ratio of Th2/Th1 cells in PB of CMML patients stratified based plasma resistin low (N=4) and high levels (N=4). D. Percentage of Treg cells in healthy (N=8) vs CMML PB (N=13). E. Percentage of Treg cells in PB of CMML patients stratified based on resistin plasma concentration low (N=6) and high (N=7) against healthy controls. F. Schematic diagram of the normal monocytic maturation pathway and immune regulation versus dysregulated monocyte maturation and immune suppression found in CMML, caused by resistin that is released by immature granulocytes. Error bars = ± SEM; ns, no significance; **, P<0.01.

Article Snippet: The resistin concentration was measured in the PPP supernatant using the Human Resistin Standard ABTS ELISA Development Kit (Peprotech, 900-K235), according to manufacturer’s protocol.

Techniques: Ex Vivo, Clinical Proteomics, Concentration Assay